Aging and senescence induced by telomere shortening, cause endothelial dysfunction that can be restored by Tempol and Apocynin.
Senescence-Induced Oxidative Stress Causes Endothelial Dysfunction.
1Department of Pediatric Nephrology, Hepatology and Metabolic Diseases, Children’s Hospital, Hannover Medical School, Hannover D-30625, Germany. REBIRTH Excellence Cluster, Hannover Medical School, Hannover D-30625, Germany.
2Department of Nephrology, Hannover Medical School, Hannover D-30625, Germany.
Age is a risk factor for cardiovascular disease, suggesting a causal relationship between age-related changes and vascular damage. Endothelial dysfunction is an early pathophysiological hallmark in the development of cardiovascular disease. Senescence, the cellular equivalent of aging, was proposed to be involved in endothelial dysfunction, but functional data showing a causal relationship are missing. Endothelium-dependent vasodilation was measured in aortic rings ex vivo. We investigated aortas from aged C57Bl/6 mice (24-28 months), in which p16 INK4a and p19 ARF expression, markers of stress-induced senescence, were significantly induced compared to young controls (4-6 months). To reflect telomere shortening in human aging, we investigated aortas from telomerase deficient (Terc-/-) mice of generation 3 (G3). Endothelium-dependent vasodilation in aged wildtype and in Terc-/- G3 mice was impaired. A combination of the superoxide dismutase mimetic 1-Oxyl-2,2,6, 6-tetramethyl-4-hydroxypiperidine (TEMPOL) and the nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor apocynin significantly improved endothelium-dependent vasodilation in aged wildtype and Terc-/- G3 mice compared to untreated controls. We show that both, aging and senescence induced by telomere shortening, cause endothelial dysfunction that can be restored by antioxidants, indicating a role for oxidative stress. The observation that cellular senescence is a direct signalling event leading to endothelial dysfunction holds the potential to develop new targets for the prevention of cardiovascular disease.