SOD protects against oxidative stress caused by cisplatin.
Acta Otolaryngol. 2010 Apr;130(4):453-7.
Endogenous protection against oxidative stress caused by cisplatin: role of superoxide dismutase.
González-García JA1, Nevado J, García-Berrocal JR, Sánchez-Rodríguez C, Trinidad A, Sanz R, Ramírez-Camacho R.
1Servicio de Otorrinolaringología, Instituto Oncológico Kutxa-Onkologikoa, Paseo Dr Begiristain 119, San Sebastián, Guipúzcoa.
Abstract
CONCLUSION:
The administration of cisplatin induces the activation of superoxide dismutase (SOD) as a response to oxidative stress in the cochleae of Sprague-Dawley rats and this activation is proportional to the activation of the intrinsic pathway of apoptosis.
OBJECTIVES:
To determine the role of the antioxidant endogenous mechanism in the preservation of cochlear integrity and function in an experimental model of cisplatin ototoxicity.
METHODS:
Sixteen Sprague-Dawley rats were studied at 7 days after intraperitoneal injection of CDDP (n = 8) or 10 ml/kg NaCl 0.9% w/v in the control group (n = 8) by means of auditory steady-state responses. These findings were compared with the expression of SOD and caspase-3/7 and caspase-9 activities.
RESULTS:
Groups receiving cisplatin showed increased auditory thresholds after injection of cisplatin and control groups maintained normal hearing. Measurements of caspase-3/7 and caspase-9 showed a significant increase in cisplatin-treated rats. A significantly increased activity of total SOD in whole cochlear extracts was observed in animals from the CDDP groups vs control animals. Likewise, differences between CDDP groups were also statistically significant.
