Fluoroquinolone antibiotics quenched by Tempol
Spectrochim Acta A Mol Biomol Spectrosc. 2014 Dec 10;133:887-91.
Fluorescence quenching of fluoroquinolone antibiotics by 4-hydroxy-TEMPO [Tempol} in aqueous solution.
Żamojć K1, Wiczk W2, Zaborowski B2, Makowski M2, Pranczk J2, Jacewicz D2, Chmurzyński L2.
- 1University of Gdansk, Faculty of Chemistry, Wita Stwosza 63, 80-308 Gdansk, Poland. Electronic
- 2University of Gdansk, Faculty of Chemistry, Wita Stwosza 63, 80-308 Gdansk, Poland.
Abstract
The fluorescence quenching of norfloxacin, danofloxacin, enrofloxacin and levofloxacin, belonging to a group of fluoroquinolone antibiotics, by 4-hydroxy-TEMPO [Tempol] was studied in aqueous solutions with the use of steady-state, time-resolved fluorescence spectroscopy as well as UV-VIS absorption spectroscopy methods. In order to understand the mechanism of quenching the absorption and fluorescence emission spectra of all fluoroquinolone antibiotics studied as well as decreases of their fluorescence were registered as a function of the 4-hydroxy-TEMPO concentration. No deviations from a linearity in the Stern-Volmer plots (determined from both, steady-state and time-resolved measurements) were observed. The fluorescence quenching mechanism was proved to be totally dynamic, what was additionally confirmed by the registration of Stern-Volmer plots at 5 temperatures ranging from 15 to 55°C. On the basis of theoretical calculations of fluoroquinolones’ molecular radii and ionization potentials the mechanism of electron transfer was rejected. It seems that the fluorescence quenching is diffusion-limited and is caused by the increase of nonradiative processes, such as internal conversion or intersystem crossing. The Stern-Volmer quenching constants and bimolecular quenching constants were determined at the room temperature for all fluoroquinolone antibiotics studied.
