Tempol protects human lymphocytes from genotoxicity induced by cisplatin.
Int J Clin Exp Med. 2014 Apr 15;7(4):982-8
Tempol protects human lymphocytes from genotoxicity induced by cisplatin.
Khabour OF1, Alzoubi KH2, Mfady DS1, Alasseiri M1, Hasheesh TF1.
1Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, Jordan University of Science and Technology Irbid, Jordan.
2Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology Irbid, Jordan.
Abstract
The use of cisplatin in treatments of human malignancies is limited by its side effects that include DNA damage and the subsequent risk of developing secondary cancer. In this study, we examined the possible protective effect of Tempol against DNA damage induced by cisplatin in human lymphocytes using chromosomal aberrations (CAs) and sister chromatid exchanges (SCEs) assays. Cisplatin induced significant elevation in the frequencies of CAs and SCEs in cultured human lymphocytes (P < 0.01). Treatment of lymphocytes with Tempol significantly lowered CAs and SCEs induced by cisplatin. Tempol alone did not affect spontaneous levels of SCEs and CAs observed in the control group (P > 0.05). In conclusion, Tempol protects human lymphocytes against genotoxicity induced by the anticancer drug cisplatin.
